HPHC Testing
Regardless of whether your products are e-cigarettes (ENDS), e-liquids, e-vapor devices (ENDS), cigarettes, oral tobacco products, gels or tobacco free oral nicotine products, Labstat offers validated methods for every chemical on the FDA’s HPHC list (Harmful and Potentially Harmful Constituents). Our validation process is designed to meet regulatory standards to ensure that submitted data is reliable and defensible.
HPHC Testing Services
| Group | Instrument | Analytes |
|---|---|---|
| Aromatic Amines | GC-MS (NCI) or GC-MS | 1-aminonaphthalene, 2,6-dimethylaniline, 2-aminonaphthalene, 4-aminobiphenyl, o-anisidine, o-toluidine |
| Caffeic acid | HPLC-UV | caffeic acid |
| Carbonyls | GC-MS (SIM) | acetaldehyde, acetone, acrolein, crotonaldehyde, formaldehyde, methyl ethyl ketone, propionaldehyde, |
| Nicotine degredation products | LC-MS/MS | anabasine, nornicotine |
| Aromatic amines, heterocyclic | LC-MS/MS | 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ), 2-amino-3-methyl-9H-pyrido[2,3-b]indole (MeAαC), 2-amino-6-methyldipyrido[1,2-a:3’,2’-d]imidazole (Glu-P-1), 2-amino-9H-pyrido[2,3-b]indole (AαC), 2-aminopyrido[1,2-A:3’,2’-D]imidazole (Glu-P-2), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b] indole (Trp-P-1), 3-amino-1-methyl-5H-pyrido[4,3-b] indole (Trp-P-2) |
| Hydrogen cyanide | CFA | HCN impinger, HCN pad, total HCN |
| Metals, mercury | CVAA | mercury |
| Hydrazine | LC-MS/MS | hydrazine |
| Metals | ICP-MS | arsenic, beryllium, cadmium, chromium, cobalt, lead, nickel, selenium |
| Ammonia | IC | Ammonia |
| Polynuclear aromaric hydrocarbons and aza-arenes | GC-MS (SIM) | 5-methylchrysene, dibenz(a,e)pyrene, dibenz(a,h)pyrene, dibenz(a,i)pyrene, dibenz(a,l)pyrene |
| Polynuclear aromaric hydrocarbons | GC-MS (SIM) | benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(c)phenanthrene, benzo(j)aceanthrylene , benzo(k)fluoranthene, chrysene, cyclopenta(c,d)pyrene, dibenz(a,h)anthracene, indeno(1,2,3-cd)pyrene, naphthalene |
| Phenols | HPLC-FLU | catechol, m-cresol, o-cresol, p-cresol, phenol |
| Radionucleotides | polonium-210 (sub-contracted) | |
| Volatiles, semi | GC-MS (SIM) | acetamide, acrylamide, benzo(b)furan, nitrobenzene, quinoline, styrene |
| Volatiles, semi | GC-MS (TQU) | ethyl carbamate |
| NFDPM, nicotine and CO | NFDAM, nicotine, water, carbon monoxide, TPM | |
| Nitrosamines, tobacco specific | LC-MS/MS | 4-[methyl(nitroso)amino]-1-(3-pyridinyl)-1-butanone (NNK), N-nitrosonornicotine (NNN) |
| Radionucleotides | uranium-235, uranium-238 (sub-contracted) | |
| Nitrosamenes, volatile | LC-MS/MS | 1-nitrosopiperidine (NPIP), N-nitrosodiethanolamine (NDELA), N-nitrosodiethylamine (NDEA), N-nitroso-dimethylamine (NDMA), N-nitrosomorpholine (NMOR), N-nitroso-N-methylethylamine (NEMA), N-nitrosopyrrolidine (NPYR |
| Volatiles | GC-MS (SIM) | 1,3-butadiene, acrylonitrile, benzene, ethylbenzene, ethylene oxide, furan, isoprene, nitromethane, propylene oxide, toluene, vinyl acetate, vinyl chloride |
| Volatiles | GC-TEA | 2-nitropropane |